Coccidia is a protozoan parasite. Coccidiosis, that malady caused by Coccidia, can be one of the most economically devastating diseases in many livestock. It can be especially harmful to recently weaned kids. It causes a watery diarrhea that is sometimes bloody and can even be a life-threatening problem to an especially young animal. The presence of Coccidia in the intestines of an individual does not mean the animal is actually suffering from coccidiosis as Coccidia are found everywhere. These protozoans only cause disease when their numbers become so great that damage is done to the host.
Some species of Coccidia found in animals:
- Eimeria - goats, swine, horses, cattle, sheep, poultry, rabbits
- Toxoplasma - cat family only
- Isospora - dogs, cats, primates, swine
- Neospora Caninum - dogs
- Sarcocystis - carnivores and omnivores
- Cryptosporidium - broad host spectrum including humans
- Mix up the flotation solution. It should be saturated. This means that you dissolve as much solid in the water as it will hold. You can use a variety of chemicals including salt or sugar. Saturated sugar is prepared by dissolving a pound (454 grams) of sugar in 1 1/2 cups (355 ml) of water, and saturated salt takes a pound (454 grams) of salt in 4 4/5 cups (1140ml ) of water. If there are salts left in the bottom of the liquid, pour off the saturated liquid into a new container.
- Collect fresh feces. Use an old pill bottle or a small jar for each animal. Be sure to label the container with the date, time and animal that provided the specimen.
- Place 3 or 4 fresh goat pellets (one to three grams) into a test tube and pour in just enough flotation solution to cover them.
- Mash them up in the liquid with your stirring rod. Add more of the solution and pour it through the strainer or cheesecloth to remove the large particles. Pour the strained liquid into a clean test tube.
- Fill up the test tube to the very top with more liquid. Place a microscope coverslip over the top. There should be no air between the coverslip and the liquid. Over time (20-30 minutes) the eggs will float up to the top and adhere to the glass plate.
- Carefully remove the coverslip and lower it at an angle over a microscope slide with the sample sandwiched between both pieces of glass.
- Examine the specimen for worm eggs and coccidia oocysts. Start with the lowest power (40x) on your microscope and carefully move up to 100x and even 400x if you see something interesting. An illustrated chart would be helpful in identifying the parasites. Note, you will also be looking at other debris. Do not confuse it with parasites.
- You should be able to see coccidia oocysts, nematode eggs, and some tapeworm eggs. Nematode eggs are shed by a large number of nematodes (worms), most of which cannot be easily distinguished from each other with this type of procedure. This group is referred to as strongyle eggs and worming recommendations can be based on the quantity of strongyle eggs present. Since fecal counts only estimate the parasite load, there is no clear cut level when worming should be undertaken. As a general guide, a level of about 500 eggs per gram of feces would indicate that worming is required for sheep, goats, or cattle. A better way of deciding when to treat would be to monitor fecal samples every 4-8 weeks and worm when there is a dramatic rise in egg counts.
|Coccidia oocysts in a fecal flotation from a cat. Image courtesy Joel Mills.|