Wednesday, May 16, 2018

Fluorescence Microscopy

Fluorescence microscopy is a tool used in biology, material science, and biomedical sciences. The fluorescence microscope can reveal the presence of a single molecule. The basic function of a fluorescence microscope is to irradiate the specimen with a desired and specific band of wavelengths, and then to separate the much weaker emitted fluorescence from the excitation light.

The fluorescence microscopy images shown below were captured using the Fein Optic RB30-GFP fluorescence microscope and the Lumenera Infinity 3S-1UR microscope camera.

Fein Optic RB30-GFP fluorescence microscope image.
Fluorescence microscopy image captured using a 60x objective lens.

Fein Optic RB30-GFP fluorescence microscope image from Microscope World.
Fluorescence microscope image, 600x.

Contact Microscope World with questions regarding fluorescence microscopy.

Thursday, April 19, 2018

Spring Flowers under the Microscope

In the spirit of welcoming spring, below are some photos of flowers captured under the biological microscope and stereo microscope.

Convallaria (Lily of the Valley) under a Zeiss microscope.
Convallaria (Lily of the Valley) captured with Zeiss Primostar HD microscope, 400x.

Monocot under a student microscope, 100x.

Flower captured with a stereo microscope.

Wishing you a beautiful spring!

Thursday, February 15, 2018

How to Set Up Koehler Microscope Illumination Properly

There are two key areas on a compound biological microscope with Koehler illumination that need to be adjusted in order to set up the Koehler illumination properly: field diaphragm and aperture diaphragm.

The microscope's aperture diaphragm can be found on the condenser of the microscope and it is typically a lever that is adjusted, or a built-in slider on the condenser.

Microscope aperture diaphragm
This is the RB40 microscope, the arrow points to the aperture diaphragm.
The aperture diaphragm on the microscope will usually have numbers on it that correlate to the numerical aperture (N.A.) on your objective lenses.

Microscope condenser aperture diaphragm
Aperture diaphragm on the microscope condenser.

Setting up Koehler Illumination

  1. Start by adjusting the field diaphragm (on the bottom of the microscope where the light is). Focus on your image and open the field diaphragm all the way up so lots of light shines through.
  2. Close the aperture diaphragm (on the condenser) down so you can see the edges of it. Is this dark circle centered in your field of view? If not, use the small centering screws on either side of the condenser to move the circle into the center. 
  3. Open the aperture diaphragm back up until the image contrast is sharp and the edges of the aperture diaphragm are crisp and clear.
This video using the Motic BA410 microscope is a great tutorial to watch if you are uncertain of the steps to take when setting up Koehler illumination on your microscope.

You can learn more about Koehler illumination and setup here.

Wednesday, January 31, 2018

High School Microscope HSX1

Richter Optica recently introduced the new HSX1 high school microscope. This student microscope has several features that make it appealing to both teachers and students.

Richter Optica HSX1 high school microscope
HSX1 High School Microscope
This high school microscope has a head that rotates 360°, making it easy for students to share the microscope. Teachers like the wider base to help avoid tip-over risk and the locked-on eyepiece. Key microscope features include:
  • Built-in mechanical stage
  • Cool LED illumination
  • Coarse & Fine focusing for crisp images
  • 40x, 100x and 400x magnification
  • Operate corded or cordless
Learn more about the HSX1 microscope here, or contact Microscope World for information about other high school microscope options.

Thursday, January 11, 2018

Protists under the Microscope

Protists are any member of a group of diverse eukaryotic unicellular microscopic organisms. Protist typically refers to a eukaryote that is not a true animal, plant or fungus because it lacks a multicellular stage.

In order to view protists, a biological microscope is required with 400x magnification.

The image below of a protist was captured using a sample of pond water under the Zeiss Primostar HD digital microscope.

Image of a Protist captured under the Zeiss Primo Star Digital Microscope (c) Microscope World
Protist captured under a digital biological microscope.

For more information on protists visit Rutgers University page.

Contact Microscope World with microscopy questions.

Wednesday, November 29, 2017

Fluorescence Microscopy

Fluorescence microscopy is an essential tool in biological and biomedical sciences, as well as in material science. The fluorescence images below were captured using a research microscope with a research microscopy camera.

Green fluorescence microscopy image
Green fluorescence image captured with a research microscope.

Monochrome fluorescence microscopy image
Monochrome fluorescence image captured with a research microscope.

Fluorescnece microscopy image showing multi channel fluorescence.
Multi channel fluorescence microscopy image captured with a research microscopy camera.

For more information on fluorescence research microscopes or research microscopy cameras for your specific application contact Microscope World.

Thursday, November 9, 2017

Tissue Biopsy under the Microscope

A biopsy is a way for a doctor to diagnose a disease. A sample of tissue or cells is removed from the patient by a doctor and sent to a pathologist to be examined under the microscope.

The following images were captured under a pathology microscope during a tissue biopsy.

Microscopy image of tissue biopsy.
Tissue biopsy captured under a pathology microscope.

Microscopy image of tissue captured under a pathologist microscope.
Tissue biopsy captured under a pathology microscope.

Tissue biopsy captured under a pathologist's microscope.
Tissue biopsy captured under a pathology microscope.

Tissue biopsy captured under a pathologist's microscope.
Tissue biopsy captured under a pathology microscope.

To learn more about different types of biopsies click here. For more information on pathology microscopes contact Microscope World.