Thursday, April 19, 2018

Spring Flowers under the Microscope

In the spirit of welcoming spring, below are some photos of flowers captured under the biological microscope and stereo microscope.

Convallaria (Lily of the Valley) under a Zeiss microscope.
Convallaria (Lily of the Valley) captured with Zeiss Primostar HD microscope, 400x.

Monocot under a student microscope, 100x.

Flower captured with a stereo microscope.

Wishing you a beautiful spring!

Thursday, February 15, 2018

How to Set Up Koehler Microscope Illumination Properly

There are two key areas on a compound biological microscope with Koehler illumination that need to be adjusted in order to set up the Koehler illumination properly: field diaphragm and aperture diaphragm.

The microscope's aperture diaphragm can be found on the condenser of the microscope and it is typically a lever that is adjusted, or a built-in slider on the condenser.

Microscope aperture diaphragm
This is the RB40 microscope, the arrow points to the aperture diaphragm.
The aperture diaphragm on the microscope will usually have numbers on it that correlate to the numerical aperture (N.A.) on your objective lenses.

Microscope condenser aperture diaphragm
Aperture diaphragm on the microscope condenser.

Setting up Koehler Illumination

  1. Start by adjusting the field diaphragm (on the bottom of the microscope where the light is). Focus on your image and open the field diaphragm all the way up so lots of light shines through.
  2. Close the aperture diaphragm (on the condenser) down so you can see the edges of it. Is this dark circle centered in your field of view? If not, use the small centering screws on either side of the condenser to move the circle into the center. 
  3. Open the aperture diaphragm back up until the image contrast is sharp and the edges of the aperture diaphragm are crisp and clear.
This video using the Motic BA410 microscope is a great tutorial to watch if you are uncertain of the steps to take when setting up Koehler illumination on your microscope.

You can learn more about Koehler illumination and setup here.

Wednesday, January 31, 2018

High School Microscope HSX1

Richter Optica recently introduced the new HSX1 high school microscope. This student microscope has several features that make it appealing to both teachers and students.

Richter Optica HSX1 high school microscope
HSX1 High School Microscope
This high school microscope has a head that rotates 360°, making it easy for students to share the microscope. Teachers like the wider base to help avoid tip-over risk and the locked-on eyepiece. Key microscope features include:
  • Built-in mechanical stage
  • Cool LED illumination
  • Coarse & Fine focusing for crisp images
  • 40x, 100x and 400x magnification
  • Operate corded or cordless
Learn more about the HSX1 microscope here, or contact Microscope World for information about other high school microscope options.

Thursday, January 11, 2018

Protists under the Microscope

Protists are any member of a group of diverse eukaryotic unicellular microscopic organisms. Protist typically refers to a eukaryote that is not a true animal, plant or fungus because it lacks a multicellular stage.

In order to view protists, a biological microscope is required with 400x magnification.

The image below of a protist was captured using a sample of pond water under the Zeiss Primostar HD digital microscope.

Image of a Protist captured under the Zeiss Primo Star Digital Microscope (c) Microscope World
Protist captured under a digital biological microscope.

For more information on protists visit Rutgers University page.

Contact Microscope World with microscopy questions.

Wednesday, November 29, 2017

Fluorescence Microscopy

Fluorescence microscopy is an essential tool in biological and biomedical sciences, as well as in material science. The fluorescence images below were captured using a research microscope with a research microscopy camera.

Green fluorescence microscopy image
Green fluorescence image captured with a research microscope.

Monochrome fluorescence microscopy image
Monochrome fluorescence image captured with a research microscope.

Fluorescnece microscopy image showing multi channel fluorescence.
Multi channel fluorescence microscopy image captured with a research microscopy camera.

For more information on fluorescence research microscopes or research microscopy cameras for your specific application contact Microscope World.

Thursday, November 9, 2017

Tissue Biopsy under the Microscope

A biopsy is a way for a doctor to diagnose a disease. A sample of tissue or cells is removed from the patient by a doctor and sent to a pathologist to be examined under the microscope.

The following images were captured under a pathology microscope during a tissue biopsy.

Microscopy image of tissue biopsy.
Tissue biopsy captured under a pathology microscope.

Microscopy image of tissue captured under a pathologist microscope.
Tissue biopsy captured under a pathology microscope.

Tissue biopsy captured under a pathologist's microscope.
Tissue biopsy captured under a pathology microscope.

Tissue biopsy captured under a pathologist's microscope.
Tissue biopsy captured under a pathology microscope.

To learn more about different types of biopsies click here. For more information on pathology microscopes contact Microscope World.

Thursday, October 19, 2017

Understanding Kidney Disease in Dogs

Kidney failure can cause of death in dogs and there are a number of different ways that kidney disease takes shape in dogs. Veterinarians use a microscope to test for some of the following problems in dogs:
  • Glomerular Disease: This is damage to the kidney filters and can be caused by infections such as Lyme disease or cancer.
  • Pyelonephritis: This is an infection of kidney tissues. If a veterinarian finds this infection, the bacteria that is causing the inflammation of kidney tissues can often be killed.
  • Neophrolithiasis: These are kidney stones. Unlike in humans, kidney stones actually do not typically cause dogs too much pain in the early stages.
  • Ureteral Obstruction with Hydronephrosis: This is a kidney blockage. If a dog has kidney stones and they fragment, they can cause a kidney blockage that will not allow urine to pass and the kidneys will swell and become damaged.
  • Tubulointerstitial Disease: This is damage to kidney tabules, often with an unknown cause. This type of kidney disease can only be determined by using a microscope to examine a kidney biopsy (see image below). 
  • Leptospirosis: This is a bacterial infection and can cause kidney disease along with organ problems in both people and dogs. Infection can be caused by contact with urine or other bodily fluids from the infected dog.
  • Toxins: These are medications, household chemicals, and even ordinary food like grapes or raisins. If your pet ingests toxins contact ASPCA Poison Control immediately.
  • Cancer: The good news is that kidney cancer is not common in dogs. The bad news is that treatment options for kidney cancer in dogs is quite limited. If only one kidney is affected it can be removed with a positive outcome. If the cancer is benign or has not spread the outcome can be good as well.
  • Amyloidosis: This is a protein issue with the kidneys where protein deposits replace normal tissue. 
  • Hereditary: Some purebred dogs have a higher rate of kidney disease than others. Additionally, some young dogs will fail to develop normal kidneys.
The image below is of a dog kidney and was captured using a veterinary lab microscope with a microscope camera.

Dog kidney captured under a veterinarian's microscope.
Dog Kidney under the Veterinary Microscope

For more information about veterinarian microscopes contact Microscope World.