The condenser should be centered for best results. These are the several ways to center your microscope condenser. Typically the condenser has adjustment screws on either side that allow for centering.
- The center of the top lens can be marked with a small ink dot and centering is then performed by making adjustments to bring it into alignment with the optical axis.
- If the substage iris is attached to the condenser (this is common), the centering can be accomplished by observing the image of the closed iris with a low power microscope objective.
The substage iris diaphragm should be adjusted to allow the most amount of light possible without over-exposing the specimen. An iris diaphragm that is open too far typically results in an image that does not have ideal resolution.